
Hi Kane,
I would be concerned (whatever the stud fee) at 2 matings so early in the season without premate tests etc, especially as she has started showing colour again. The blood tests will let you know if she has/has not yet ovulated or is currently ovulating - prices vary between vets, but are well worth it. Check with the dog owner to see how many matings you are allowed.
We recently had a bitch who showed signs of being ready on day 9, and was mated days 9 & 11. Day 15 she started flagging her tail much more strongly and flirting desperatly with anything in sight,, dog or bitch :). Mated her again, and whilst she had been reasonably willing for the first 2 matings she was a whole lot more keen for this one.
Is this your first litter from this bitch? It can be easier for subsequent matings when you have an idea of the bitches season and fertility pattern.
bye
Gwen