We have been planning our mating one of our dogs (maiden) to one of our bitches (also a maiden) for many many months. Her season was 8.5 weeks late in coming, last Friday (day 11 of season) I took her to the vet and he performed a blood test for ovulation. He said that the results were inconclusive, she could be ready on Monday/Wednesday (days 14 & 16), however it would be best to try her on Sunday and if no joy, come back again for a further test on Monday.
On Sunday, things looked very promising, lots of tails to one side, and he was not afraid to jump on, she would then proceed to walk (slowly), hence was not totally standing still for him, he could not steady himself enough to make contact. lots of happy playing, all looked good. We did try to steady her, just by gently holding her collar, but he clearly liked her to be free as he immediaetly jumped off when anyone was holding her. Without steadying her there was not a hope, so we called it a day.
On Monday morning, all was much the same. Took her back to the vets at 4pm, they rang me 45 minutes later and said test much better this time, and she would definately be ovulating on Wednesday & Friday (days 16 & 18) this week, we discussed how relieved I was that she had not missed. Again, he said to try them together late on Tuesday to see how they were.
We tried them together on Tuesday evening, but she was very growly, something we had not had previously, so we seperated them and left it at that.
This evening, we have put them together and she is awful (she's not going to bite him, of that I am confident) however, she is pretty wicked sounding (and normally the sweetest natured!) He is not taking it personally, however stops what he is doing as soon as she starts up. He has had one or two go's back at telling her off, and she has demonstrated totally submissive behaviour towards him. Though tonight we have no jumping on, he has tried twice and each time she has growled etc. If I hold her collar, he does not want to know, we go back to the being free thing! Her tail is not swinging to the side so much (very little in fact!)
If I try to distract her, talking to her, toys whatever, he leaves the back end investigations and come over to the front end to see what is happening!
HELP!!!

I do have utter faith in my vet, though realise that human error can be made, I am not sure if she's missed, or if she just does not want to be mated!
Suggestions please, needless to say, I will call the vet again first thing tomorrow morning to see what he has to say, though I am sure he will just suggest another test, which I am likely to take him up on. We are planning on putting them together tomorrow morning again and seeing what they are like.
If we have missed, I would rather know, as it seems wrong to keep putting them together, however I don't want to miss my chance if we haven't missed.
I am totally torn and not sure what to think. By the way I have had the assistance of a very experienced breeder, I myself am a novice and only have one litter every two-three years.
Do you think we have missed? if not, any suggestions on what I can do?.....
Thanks for all suggestions, opinions and whatever you can offer to help
Candy