
Radiography
Radiographic imaging (X-ray) of the abdomen is most accurate for determining pregnancy during the last trimester (43 to 63 days after ovulation). After 42 days, the fetal skulls and spines are visible on radiographs. As pregnancy advances, the bones of the front legs become visible, followed by the bones of the rear legs, the pelvis and the ribs. Finally, the fetal teeth become visible around 56 to 61 days after ovulation.
Prior to 40 days, the enlarging uterus may be visible on radiographs, but it may appear very much like the surrounding intestines. The contents of the pregnant uterus may be difficult to distinguish from an abnormal non-pregnant uterus before fetal skeletons develop. The physical and medical condition of the bitch will help to identify a diseased or a healthy pregnant uterus.
As the pregnancy nears its end, the number of fetuses can be determined radiographically by counting fetal skulls. Brachycephalic breeds (English bulldogs, Boston terriers, boxers, etc.) have an increased risk for difficult deliveries due to the size and shape of the fetal skulls. Radiographic evaluations late in pregnancy will determine if the fetuses are developed sufficiently (teeth visible) to survive and whether or not a cesarean section should be performed.