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Has anyone had progesterone testing with axiom? What scale do they work with?
By roxx
Date 26.01.15 11:20 UTC
According to their web site its
OVULATION DETECTION IN BITCHES
This involves sequential heparinised plasma or serum samples for progesterone.
Most bitches ovulate on day 13 of the cycle, it is recommended that basal levels are established on days 9-10, then the
lab will advise on when the next sample should be taken, based on the following :
<6 nmol/L NOT OVULATED
6-10 nmol/L OVULATED MATE WITHIN 72 HOURS
10-20 nmol/L OVULATED MATE WITHIN 48 HOURS
20-32 nmol/L OVULATED MATE WITHIN 24 HOURS
32 nmol/L TOO LATE
It is wise to note that these values are usually reported 24 hours post sampling and those 24 hours need to be taken off
the above times, i.e. the above times relate to the time of sampling.

Oh you found it. Clearly I was too blind to see. Thanks. Although in the end we ended up using Keith and then capl
By roxx
Date 06.02.15 08:52 UTC
yes, i also contacted them and they only offer the service to vets not to the general public.
Thanks for posting this table. I have previously used IDEXX for testing and their range for mating seems to differ somewhat from this one? i.e. this says over 32 is too late whereas IDEXX says over 90 is too late, which seems like a big difference!
I am currently testing a bitch with a vet that is sending off to CAPL, and I am interpreting the readings according to what I have always thought with IDEXX, but should I be bearing in mind this range instead?
By dogsbody100
Date 06.02.15 12:54 UTC
Edited 06.02.15 12:57 UTC
By kennel maiden
Date 06.02.15 13:03 UTC
Edited 06.02.15 13:14 UTC
> kennel maiden - the table given by roxx is nmol/l and I believe Idexx give their levels in ng/ml, a totally different progesterone levels table.<br />
Really, I always thought IDEXX was given in nmol too??!! I have always asked and written them down as such.... now, very confused!
I've asked what the CAPL readings are, and they have also said nMol. I just want to know if I can compare them like for like with IDEXX, or whether they are more akin to the Axiom scale that is detailed here? I had never considered that there may be different scales using the same units before. That doesn't really make sense to me. I could understand it, though, as you say, if one was actually in ng/ml, but I don't think that is the case?
I've cut and paste this from my previous post about missing ovulation. Somebody kindly posted this table of progesterone assay figures:
Here are the numbers:
0-2 nmol/L Baseline concentrations too early to estimate ovulation
3 - 6 nmol /L Minimum 2 days before ovulation expected however result of 3 - 4 nmol/L may persist for a week or longer before increasing. Earliiest estimating breeding 4 - 6 days but could be longer (re test needed)
7 - 12 nmol/L Minimum 1 day before ovulation. Estimated window for breeding 3 - 5 days but could be longer ( re test recommended)
13 - 18 nmol/L Ovulation impending or just occurred. Estimated breeding window 2 - 4 days
19 - 31 nmol/L Ovulation recently occurred. Estimated breeding window 1 - 3 days
32 - 64 nmol/L Ova have matured, optimal potential for fertility. Estimated breeding window 0 - 2 days
65 - 90 nmol/L Ova have matured but aging, decreased potential for fertility. Breed at once
Above 90 nmol/L Too late
I presumed this was from IDEXX (as I have used them before and this would relate to the figures they gave me). So, ovulation occurs for argument's sake around 15nmol, but on the Axiom chart they are saying it occurs much sooner at 6-10 nmol and everything is over by 32 (whereas IDEXX is saying mate at 32?!) ??
You are indeed correct that Idexx use nmol/l. As no poster here is qualified to answer your question (except Paris, who does not seem to be around at the moment) suggest you email the Laboratories concerned and get the definitive answer.
Thanks, yes I phoned CAPL again to double, double check as was starting to worry I had got it wrong

, but they are in nMol

, as is IDEXX and Axiom also says they are nMol! Their ovulation readings are similar/ballpark, it is just what they consider to be past/over that is so very different. With IDEXX you would still be happy to mate and keep trying at 35nMol, whereas Axiom are saying no point!!??

I think axiom are saying no point at mating past 35nmol Because you get the results the day after so you'd be at least 70nmol when you get the results for the bloods from the day before. I don't know, it's just a guess. Everyone I've ever spoken to would say that at 35 you still have a small window of opportunity
By Paris
Date 06.02.15 14:32 UTC
Upvotes 1
Hi
All commercial laboratories tend to have their machines set to read in N MOLS >The machine that is commonly used is the siemens immulite ,which will give the result in either N MOLS or NG/ML depending on how you wish to have it set up. The two scales are just like cm and metres or feet and inches : where a 10 cm on idexx machine will be the same as a 10 cm on anybody elses machine
I personally have my machine set to read in NG/ML ,but when we report the result to a customer we give them the result in both nmols and ng
a result of 35nmols would be the equivalent of 11.006ng and would be ok for a natural mating but far too early for a frozen semen insemination
hope this helps
Absolutely! I had a bitch brought to me at 35nMol, and she had one good mating, followed by another two days later and went on to have a good sized litter. According to IDEXX she was bang on.
Luckily with CAPL, bloods are tested the same day, with results back by 330pm, so minimum delay.
By roxx
Date 10.02.15 15:44 UTC
Whenever i have used IDEXX i have always had results in nmol
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