
I'd take it from the date of birth. And be aware, if your puppies are in quite strong light, their eyes opening may be delayed. If in subdued light they do tend to start to open at the 'text book' age - around 10 days. Dogs don't read textbooks however. So wait. And whatever you do, not try to force this - if you check by around 10 days on, you may see little slits beginning to open. What you do need to check on is whether, on opening the eyes are at all cloudy. Bottom line - if concerned check with your mentor, or vet.